Human Gene Expression analysis required RNA samples with high integrity, namely an RNA quality index of 8. Gene expression analysis: The Affymetrix GeneChip® PrimeView™ Human Gene Expression Arrays were used to measure the gene expression profile of selected cancer cell lines after treatment with various extracts.
Phrase profile of eukaryotic translation factors in human being cancer tissues and cell lines.
Joseph-P; O'Kenrick-CM; Othumpangat-S; Lei-YX; Yuan-BZ; Ong-TM
Mol Carcinog 2004 Jul; 40(3):171-179
20025264
Several studies have exhibited the overexpression of particular eukaryotic translation elements in human cancer cell lines and in malignant tissues. In this research, with human cancer cell lines made from lungs, breast, prostate, and pores and skin, we have examined the expression profile of 36 translation elements containing of 27 initiation aspects, 8 elongation factors, and 1 termination aspect. Translation initiation elements 2C2 and 4E1 and translation elongation factors 1A2 and 1 had been discovered overexpressed (2- to 2000-collapse) in many of the cancer cell lines compared to their matching regular cell lines. Among the translation aspects analyzed, translation elongation element 1A2 displayed the nearly all significant change in expression: 10- to 2000-fold overexpression was noticed in nine out of ten cancer cell lines examined. Whether the overexpression of translation elongation element 1A2 can end up being utilized as a potential tumor marker was examined with the cancer profiling selection (BD Biosciences, Palo Alto, CA) consisting of 241 combined cDNA examples created from 13 various cancer/noncancer tissue types. Overexpression of translation elongation factor 1A2 has been noticed in various tumor cells samples, most notably in the human being digestive tract cancer samples which showed at least a twofold overexpression among 35% of the samples examined. Besides digestive tract, tumor examples made from lungs, kidney, rectum, and ovary also exhibited more than a two fold overexpression of translation elongation factor 1A2 in at least 20% of the examples analyzed. These results show that individual carcinogenesis can be often related with alterations in the expression of different translation elements specifically the overexpression of eukaryotic translation elongation aspect 1A2.
Cancers; Cancer-rates; Malignancy; Cell-growth; Cell-function; Cellular-reactions; Cellular-function; Cellular-structures; Carcinogens; Carcinogenicity; Carcinogenesis
Pius Joseph, MS 3014, Molecular Carcinogenesis Lab, National Company for Occupational Basic safety and Health, 1095 Willowdale Road, Morgantown, WV 26505.
MOCAE8
20040601
Paper Post
2004
3
0899-1987
HELD
Research Equipment and Methods: Tumor Research Methods
Molecular Carcinogenesis
WV